Encoding a 20-kDa Protein
نویسندگان
چکیده
The amino acid sequence of a noyel trypsin inhibitor (p20) was completed by the molecular cloning of the protein in cultured soybean ce)ls. The clone nucleotide contains an open reading frame encoding a polypeptide of 206 amino acids that shows 45-50% sequence homology to members of the Kunitz-type trypsin inhibitor fatnily. The p20 transcript is expressed in the roots, stems and )eayes ef soybean seedlings. DNA gel blot analyses show that the p20 in soybean is encoded by ft single gene, and that this gpne may not contain an
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